PolyPen
Quick, reliable and easy measurements in the field or laboratory
About...

The PolyPen RP 410 is a sophisticated handheld instrument that incorporates an internal Xenon incandescent lamp and measures spectral reflectance of leaves between 380 - 1050 nm.

The PolyPen RP 410 firmware calculates formulas of commonly used reflectance indexes (e.g. DVI, NDGI, PRI etc.) and displays these on its touch screen instantaneously as graphs or data sheets. All data are stored as full spectra in the Polypen's memory and are available for customised calculations and transfer to a PC.

Key Features

  • Complete system for measurement of spectral reflectance of leaves
  • Automatic calculation of all commonly used reflectance indices
  • Calculation of customised indices
  • Rugged, compact and battery-powered for lab and field applications
  • Programmable via intuitive touch-screen


  • Integrated light source
  • Leaf clip for non-destructive, in-situ measurements
  • Comprehensive software for data processing
  • USB and Bluetooth communication for data transfer
  • Integrated GPS module
  • Handheld, lightweight and affordable

PolyPen RP 410

PolyPen RP 410 features a complete system for measurement of spectral reflectance of an internal light source (Xenon incandescent lamp 380 - 1050 nm) from leaves.

Polypen RP 410 incorporates formulas of commonly used reflectance indexes (e.g. DVI, NDGI, PRI etc.) into its software and displays values of selected indexes for the measured sample. Measured data are instantly displayed in graphs or data sheets on the device screen display. They are also stored as full spectrum in the device memory for later re-collection or transfer onto a PC.

PolyPen RP 410 is a self-contained system that runs on Li-Ion rechargeable battery and does not require any external PC. It features an innovative user interface with integral computer, full color touch screen display and built-in light source. The PolyPen is equipped with a non-destructive leaf-clip sample holder that has two inter-changeable adapters with reference standard for calibration of the light source and detector.


POLYPEN MEASURES


Absorbance - calculated data using the following formula
A=log(I0/I), where I0 is reference light intensity and I is measured light intensity

Transmittance - calculated data using the following formula
T= I/ I0, where I0 is reference light intensity and I is measured light intensity

LIST OF CALCULATED VEGETATION INDICES


  • Normalized Difference Vegetation Index (NDVI)
  • Reference: Rouse et al. (1974) Equation: NDVI = (RNIR - RRED ) / (RNIR + RRED )
  • Simple Ratio Index (SR)
  • Reference: Jordan (1969); Rouse et al. (1974) Equation: SR = RNIR / RRED
  • Modified Chlorophyll Absorption in Reflectance Index (MCARI1)
  • Reference: Haboudane et al. (2004) Equation: MCARI1 = 1.2 * [2.5 * (R790- R670) - 1.3 * (R790- R550)]
  • Optimized Soil-Adjusted Vegetation Index (OSAVI)
  • Reference: Rondeaux et al. (1996) ) Equation: OSAVI = (1 + 0.16) * (R790- R670) / (R790- R670 + 0.16)
  • Greenness Index (G)
  • Equation: G = R554 / R677
  • Modified Chlorophyll Absorption in Reflectance Index (MCARI)
  • Reference: Daughtry et al. (2000) Equation: MCARI = [(R700- R670) - 0.2 * (R700- R550)] * (R700/ R670)
  • Transformed CAR Index (TCARI)
  • Reference: Haboudane et al. (2002) Equation: TSARI = 3 * [(R700- R670) - 0.2 * (R700- R550) * (R700/ R670)]
  • Triangular Vegetation Index (TVI)
  • Reference:Broge and Leblanc (2000) Equation: TVI = 0.5 * [120 * (R750- R550) - 200 * (R670- R550)]
  • Zarco-Tejada & Miller Index (ZMI)
  • Reference: Zarco-Tejada et al. (2001) Equation: ZMI = R750 / R710
  • Simple Ratio Pigment Index (SRPI)
  • Reference: Peñuelas et al. (1995) Equation: SRPI = R430 / R680
  • Normalized Phaeophytinization Index (NPQI)
  • Reference: Barnes et al. (1992) Equation: NPQI = (R415- R435) / (R415+ R435)
  • Photochemical Reflectance Index (PRI)
  • Reference: Gamon et al. (1992) Equation: PRI = (R531- R570) / (R531+ R570)
  • Normalized Pigment Chlorophyll Index (NPCI)
  • Reference: Peñuelas et al. (1994) Equation: NPCI = (R680- R430) / (R680+ R430)
  • Carter Indices
  • Reference: Carter (1994), Carter et al. (1996) Equation: Ctr1 = R695 / R420; Ctr2 = R695 / R760
  • Lichtenthaler Indices
  • Reference: Lichtenthaler et al. (1996) Equation: Lic1 = (R790 - R680) / (R790 + R680); Lic2 = R440 / R690
  • Structure Intensive Pigment Index (SIPI)
  • Reference: Peñuelas et al. (1995) Equation: SIPI = (R790- R450) / (R790+ R650)
  • Gitelson and Merzlyak Indices
  • Reference: Gitelson&Merzlyak (1997) Equation: GM1 = R750/ R550; GM2 = R750/ R700)

APPLICATIONS


  • Plant screening & field studies
  • Stress response
  • Pigment composition
  • Water content of plants
  • Nitrogen status
  • Grain yield

SOFTWARE


  • Automatic calculation of all commonly used reflectance indices
  • Calculation of custom indices (possible on request)
  • Instant data graphs and data sheets
  • Export to a PC via USB and Bluetooth communication
  • Data browsing and data averaging
  • Visualization and data transfer routines to Microsoft Excel
  • GPS mapping
  • PolyPen 1.0 software (Windows 2000, XP, or higher compatible)

Versions


POLYPEN RP 410 UVIS

Spectral response range: 380 to 780 nm.

POLYPEN RP 410 NIR

Spectral response range: 640 to 1050 nm.


Specifications

Light Source: Xenon incandescent lamp 380-1050 nm

Spectral Response Range:
380 nm - 790 nm
640 nm - 1050 nm

Spectral Response Half Width: 8 nm

Spectral Straylight: -30 dB

Size of Aperture: 7 mm

Scanning Speed: About 100 ms

Touch Screen: 240 x 320 pixel; 65535 colors

Memory Capacity: 32 Mbit (up to 8000 measurements)

System Data: 16 bit AD conversion

Dynamic Range: High gain: 1:4300; Low gain: 1:13000

Communication: USB and BT

Dimension / Weight: 15 x 7.5 x 4 cm / 300 g

Case: Splash-proof

Battery: Li-Ion; rechargeable via USB port of a PC

Accessories

Mini-Computer

Small, portable notebook (type according to current availability on the market).

Spectralon Reflectance Standard

For the highest diffuse reflectance values - reflectance value 99 %. Spectrally flat over UV-VIS-NIR spectrum. Optically flat to +/- 1 % over the photopic region of the spectrum.

Self-Adhesive Rubber Pads

One set includes 10 solid pads (to be attached to the clip) and 10 pads with an opening (to be attached at the optical head).

Pricing


PolyPen

Version Characteristics Price
PolyPen RP 410/UVIS Handheld, battery-powered device for measurement
of spectral reflectance on flat leaves. Spectral response range: 380 to 780 nm.
Black and white reference standards and inbuilt GPS module are included in the price.
3,999 €
PolyPen RP 410/NIR Handheld, battery-powered device for measurement
of spectral reflectance on flat leaves. Spectral response range: 640 to 1050 nm.
Black and white reference standards and inbuilt GPS module are included in the price.
3,999 €

Accessories

Mini-Computer * Small, portable notebook (type according to current availability on the market). 390 €
Spectralon Reflectance Standard * For the highest diffuse reflectance values.
Spectrally flat over UV-VIS-NIR spectrum. Optically flat to +/- 1 %
over the photopic region of the spectrum.
200 €
Self-Adhesive Rubber Pads One set includes 10 solid pads (to be attached to the clip)
and 10 pads with an opening (to be attached at the optical head).
12 €