- Where can I check FluorPen/SpectraPen software compatibility with different Windows versions?The compatibility information are in specification of each handheld device on our website in download section.
- Is there a function "RESET TO FACTORY SETTINGS" in the FluorPen/SpectraPen software?Unfortunately, there is no such function in the software. The only possibility is to reload the device firmware, which we recommend just in emergency cases and only on the recommendation of the support team.
- Are all handheld devices equipped with both communication modules - USB and Bluetooth?New versions 110 of AquaPens, FluorPens, PlantPens and LaiPen are equipped with both communication modules (AP 110, FP 110, NDVI 310, PRI 210, N 110 and LP 110).
The old versions 100 always come with just one communication module - either USB or Bluetooth.
All vesrions of SpectraPens and PolyPens are supplied always just with USB communication module.
- My computer does not recognize that the device is connected when using the USB communication. Can you help on this?For USB connection you need to have the USB driver installed in your PC. You find the driver on the installation disk (USB driver folder). If you check the Device Manager in Windows you should see the USB serial port in the device tree. In case of missing driver you may download it from website of the particular handheld device in section download. When the driver is installed correctly you should be able to connect to the device in the FluorPen software menu Setup->Device ID.
- I transferred the measured data to a PC via the FluorPen/SpectraPen software. Now I am trying to save or export it to txt format, but the Export icon at FILE menu is not functional (disabled). Do you have an advice to enable the key and its function?You need to activate the software by entering the registration serial number. You can do it in the menu Help-Register. Your serial number is on the installation flash drive in the SN.txt file. The procedure is also described in the user manual.
- I am unable to download the data from the device to the Excel spreadsheet: please, could you tell how can I do?In the Fluorpen software go to Export and export the data to txt file. Then in Excel go to Data and Import Data from Text. In this way you export data directly to Excel and process then further. For more information about text import to excel please follow Microsoft office support.
- Is it possible to export just part of measured data or all data must be exported?It is possible to export all data or one selected measurement. Mark the selected measurement by mouse before export. Then go to Export menu and choose Selected only.
- What should I do when the display on my device shows just FP-Boot and 18.104.22.168 when switched on and does not do anything after that?The firmware update hepls to solve this issue. Please contact the support team with the serial number of your device. The appropriate BXN file will be sent to you accordingly. Please note that all data will be erased from the device memory during firmware update. It is highly recommended to back up the data before starting the update. Please update your device firmware as follows:
- Connect the device to the PC
- Select menu Setup->device ID
- Select menu Setup->Update firmware from file
- Select the downloaded BXN file
Firmware updating procedure is also depicted in the Operation Manual.I have a problem with downloading data from my handheld device. When the device gets to the end (100%) of the download, it gives an Error: TFpData:parse Data0 -> unknown ID 0. And then just sits with the spinning circle and there is no way to get data off the unit.
This error means that the data in internal memory is corrupted. The reason for that could be for example power failure (battery dead or removed) during data saving procedure. If there are no important data it could be easily solved by erasing the memory. If you need the data please do following:
Please take a look into the folder where FlourPen software is installed (default is Program Files/PSI/FluorPen) and send the file error-data.bin to the support team. Make sure you have enabled “Show hidden files”.Referring to the GPS Module - what is the accuracy of the location registration?
The GPS module works with accuracy of <1.5 m in 50% of trials. We have a problem to activate the external GPS Module. How can we activate it?
Please check the Manual provided. To correctly attach the GPS coordinates, it is essential to set time correctly on the device and on the PC. It is using time zone settings from the computer so you should check that too. Also check that Garmin is set in Mass Storage mode.What is the function of cosine corrector in PAR-sensor or light sensor of SpectraPen?
Cosine corrector is used for collecting light from 180 degrees angle. The sensor itselfs has a small angle in which light is accepted. It is about 30 degrees. It is used for relative and absolute spectral intensity measurements, for emissive color applications, and for evaluation of light sources such as LEDs and lasers. Usage is optional and the corrector can be connected or disconnected very easily.
Fluorometers (AP, FP and MP)Is the FluorPen software capable of recording used light intensitis?
Yes, new versions of handheld fluorometers (AP 110 and FP 110) are capable to record information about set light intensities.In which units is measured fluorescence in handheld devices?
Fluorescence is measured in relative fluorescence units, also called arbitrary units (A.U.).What is measuring time of fluorescence protocols?
It differs for single protocols: Ft takes about 100 ms, OJIP and QY about 1-2 seconds, NPQ and LC protocols take up to few minutes.
How big is the memory in FP/MP/AP device? How many measurements can be saved in the device internal memory?
Ft: up to 149,000 measurementsQY: up to 95,000 measurementsLC1: up to 3,000 measurementsLC2: up to 3,500 measurementsLC3: up to 2,600 measurements OJIP: up to 1,100 measurementsNPQ1: up to 800 measurements NPQ2: up to 500 measurements[b]Can you explain meaning of the Flash pulse (f_pulse)?
This function serves for setting of flash pulses intensity. The flash pulses are weak pulses, which are able to induce the minimal chlorophyll fluorescence (Fo or Ft) and are crucial for PAM method. It takes only 30 µs and the maximum intensity is 3000 µmol photon.m^-2.s^-1. It means 30 µs * 3000 µmol photon.m^-2.s^-1 = 0,09 µmol photon.m^-2 per pulse is the maximal intensity of the f_pulse.How should I adjust correctly the Flash pulse (f_pulse) settings prior to fluorescence measurement?
The optimum value of f_pulse can be identified for instance with the QY measurement. Before initiating the QY measurement it is recommended to set the pulse color based on the type of measured organism, if the device allows it and intensity of F_pulse on 70%. QY measurement should be performed with dark adapted sample. If you use the cuvette version of the AquaPen, make sure that you use always fresh 4 ml of culture for each individual measurement, as the culture can be inhibited after measurement. Or wait given time period between the individual measurements to ensure relaxation of photosynthetic apparatus during the dark adaptation of the sample prior next measurement. The f_pulse setting recommended by the manufacturer is 30%. Perform QY measurement with different intensities of f_pulse and choose the f_pulse intensity at which you reach the highest QY value.
Usually is recommended to increase the intensity of f_pulse in case of sample with very low chlorophyll density. Please note that high intensities of f_pulse can cause undesirable “actinic effect” resulting in increase of F0 value - in this case F0 isn´t real dark adapted F0 and QY value will be lower. The f_pulse intensity at which the highest value of QY is reached is the optimal one for your culture.Can you explain meaning of the Super pulse (F_pulse)?
This function serves for setting intensity of the Super pulse. Super pulse is able to induce maximum chlorophyll fluorescence (Fm). 100% of intensity equals approximately 3000 µmol photon.m^-2.s^-1.
How should I adjust correctly the Super pulse (F_pulse) settings prior to fluorescence measurement?
The best way to recognize the optimum intensity of F_pulse is to perform OJIP measurement. During OJIP measurement solely F_pulse (super/saturating pulse) is used for ChlF measurement. OJIP measurement should be performed with dark adapted sample. If you use the cuvette version of the AquaPen, make sure that you use always fresh 4 ml of culture each individual measurement, as the culture can be inhibited after measurement. Or wait given time period between the individual measurements to ensure relaxation of photosynthetic apparatus during the dark adaptation of the sample prior next measurement. The F_pulse setting recommended by manufacturer is 70%. Perform OJIP measurement with different instensities of F_pulse. The F_pulse intensity at which the highest value of Fv/Fm is reached is the optimal one for your sample.[b]Can you explain meaning of the Actinic pulse (A_pulse)?
This function serves for setting intensity of actinic pulses. Actinic light is basically the ambient light in which the plants or algae are growing. 100% of intensity equals approximately 1000 µmol.m-2
New version AP 110 and FP 110 has calibrated actinic light and it is set directly in µmol.m-2
.What does it mean when the device shows "low val" on the display when measuring fluorescence?
“Low val” means low value of fluorescence signal. Samples with very low concentration of chlorophyll show low fluorescence signal. If it is possible concentrate the sample by centrifugation, filtration or sedimentation for amplification of fluorescence signal.How should I adjust the measurement when I get „over-flow“ error message during fluorescence measurement?
The „over-flow“ error message means that the chlorophyll fluorescence signal is above the range of detection. This issue can be fixed by diluting of the sample (liquid samples) or in case the sample can not be diluted (plant leaves), increase the intensity of used pulses.Why measure dark-adapted samples?
Dark adaptation is a technique recommended in some chlorophyll fluorescence measurements to assure reference point relative to various measurements. You get higher photosynthetic performance when measuring dark-adapted samples, because in dark all reactions centers of PSII relax and are ready for incoming light energy. In light-adapted sample some of the reaction centers are saturated with the ambient light and therefore cannot accept all photons of the incoming light and you get lower QY value. You could of course compare QY values of light-adapted samples, but the result could be affected by light conditions before the measurement.
• How long dark-adaptation time is recommended?
Duration of dark adaptation depends on species, in general it is between 5 and 30 minutes.Can I measure Ft and QY in presence of light (without dark condition)?
Basically all measured fluorescence parametres can be acquired either after dark adaptation or in the light adapted state. Ft refers to Fo if the leaf sample is dark-adapted (Fo refers to minimal level of fluorescence in dark adapted state). QY measured in the light is equal to Fv´/Fm´ and it provides an estimate of the PSII maximum efficiency within light-adapted material in contrast to Fv/Fm which is an estimate of the PSII maximum efficiency within dark-adapted material. Explanation of particular parameters: Fo - minimum fluorescence signal (when all PSII centres are in the open state) from dark-adapted material; Fo' - minimum fluorescence signal (when all PSII centres are in the open state) from light-adapted material; Ft - fluorescence signal at any point between Fo' and Fm', in other words instaneous fluorescence which is affected by the level of light the object is exposed to; Fm - maximum fluorescence signal (when all PSII centres are in the closed state) from dark-adapted material; Fm' - maximum fluorescence signal (when all PSII centres are in the closed state) from light-adapted material.Do the Ft values change within one measurement or between measurements? Should I do some kind of calibration or programming before measuring?
The value can change slightly (in range 10%). If the change within one measurement is bigger, it is recommended to set the f_pulse intensity lower. Strongly increasing Ft value within one measurement means, that f_pulse is too strong and causes unwanted "actinic effect" (= photochemistry starts). Fluorescence measurements do not require calibration.
Is it possible to calculate ETR from data measured?
Yes, it can be calculated by the Light Curve data using this equation: YII*PAR*0.84*0.5.
Spectrameters (SP, LM, RP and PA)Can you shortly explain the difference in between the PolyPen RP 410, SpectraPen SP 110 and SpectraPen LM 510 ?
PolyPen RP 410 measures reflectance on leaves or other plant samples. It is capable of calculation of various reflectance indices (some of them are pre-programmed). It features an integrated light source and a leaf clip. SpectraPen SP 110 is a spectrometer without spectroradiometric calibration. It features a SMA905 light fiber port. It is intended for measuring reflectance, transmitance and light wavelength. SpectraPen LM 510 is a spectroradiometrically calibrated spectrometer with integrated cosine corrector. It measures absolute values of light intensity in uW/cm2/nm and photon flux density in umol/m2/s/nm. It calculates: irradiance (uW/cm2/), PAR (umol/m2/s), illuminance (Lux), CIE1931 coordinates, CCT (L).Is there any difference in measuring absorbance and transmittance with the PolyPen and with the SpectraPen?
PolyPen is equipped with an internal light source. Thus it is a complete system for leaf reflectance measurements not requiring any additional accessories. SpectraPen is a simple spectrophotometer with no in-built light source.Can I obtain trustworthy results when measuring with PolyPen RP 410 or PlantPen if my plant samples are small, smaller than the optical entrance of these devices?
Yes it is possible to measure smaller leaves with minor limitation. The leaf clip is equipped with white coating that is used for calibration. When you use smaller leaves the white coating become "visible" for the instrument and influence the results. This can be fixed by putting some black material as a background for the leaf to cover white coating during measurement.Does the PolyPen RP 410 or PlantPen work also on monocot leaves with a prominent midvein?
These devices have been tested and used for monocot species. However it is important to remember that veins can indeed cause some interferences during the measurement and that the first leaves of same age should be cross-compared and, in an ideal case, the method should be standardized to the same part of the leaf.